Transcription and expression of bcl-x gene in two cell lines of nasopharyngeal carcinoma
Abstract:Objective:To study the transcription and expression of apoptosis-related gene bcl-x in two cell lines of human nasopharyngeal carcinoma(NPC).Methods:Total RNA was isolated from NPC cells in high differentiation(CNE-1)and low differentiation(CNE-2z)cell lines with Trizol kit.Transcription and protein level of bcl-x were detected by RT-PCR and western blot,respectively.Results:transcription and expression of bcl-xL were detected in both cell lines,while those of bcl-xS were not.Conclusion:Bcl-xLwas expressed in high and low differentiation NPC cell lines,but its expression may be unrelated to the degree of cell differentiation.
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Publication Date:2001-01-01
Online Publishing Date:2025-08-15(First online date of this platform, not the publication date of the document)
Pages:3( 10-12 )
Journal of Guangdong Medical College

Journal of Guangdong Medical College

ISSN:1005-4057
Year, Vol.(Issue):2001,19(1)