Application of E-cadherin combined with elastic fiber staining in pleural invasion of lung adenocarcinoma

WEI Li-hua; KANG Kai-fu; YE Ying; LI Jia-tian; WENG Zhen-lin

doi:10.13820/j.cnki.gdyx.20250408

Abstract：Objective To explore the diagnostic value of immunohistochemical E-cadherin combined with elas-tic fiber staining in detecting pleural invasion of lung adenocarcinoma.Methods Fifty lung adenocarcinoma samples ad-jacent to the visceral pleura were selected.Hematoxylin and eosin(HE)staining,elastic fiber staining,and E-cadherin combined with elastic fiber staining were performed.The accuracy of pleural invasion assessment using the three staining methods was compared.Results In the combined E-cadherin and elastic fiber staining,adenocarcinoma cells exhibited brown-yellow positivity,while elastic fibers were stained Victoria blue,clearly demonstrating the relationship between adenocarcinoma cells and the visceral pleural elastic layer.Single HE staining could not display elastic fibers,and single elastic fiber staining did not clearly highlight adenocarcinoma cells,affecting accurate assessment of pleural invasion.The positive detection rates of pleural invasion in single HE staining and single elastic fiber staining groups showed no signifi-cant difference(P＞0.05),whereas the dual staining method significantly increased the positive detection rate(P＜0.05).Conclusion Compared with single HE staining or elastic fiber staining,E-cadherin combined with elastic fiber staining allows faster and more accurate assessment of visceral pleural invasion in lung adenocarcinoma,providing a new alternative method for pathological evaluation.