Research progress on the effects and influencing factors of repeated cryopreservation-thawing on human sperm
MA Chun-jie
ZHANG Xin-zong
LUO Lu-lu
Abstract：Repeated sperm cryopreservation is an effective approach to maximize the utilization of limited sperm resources,offering a valuable option for men who can no longer provide sperm.The cry opreservation of any viable sperm is worthwhile,as intracytoplasmic sperm injection(ICSI)for each oocyte requires only a single sperm cell.After the first thawing of frozen semen for use in assisted reproductive technology(ART),motile sperm often remain available.This re-view summarizes the detrimental effects of repeated freeze-thaw cycles on human sperm motility,viability,morphology,and DNA integrity,as well as the influencing factors,aiming to provide references and recommendations for the reuse and re-cryopreservation of surplus sperm in ART.
Keywords：human spermrepeated cycles of thaw-refreezingassisted reproductive technology
Publication Date：2025-09-15
Online Publishing Date：2025-11-06(First online date of this platform, not the publication date of the document)
Pages：5( 1281-1285 )