The predictive value of serum miR-181-5p,miR-21-3p,and miR-124-3p in sepsis patients complicated by acute respiratory distress syndrome
JIN Bo
CUI Yong-jun
HAN Bin
QU Wei-wei
LI Bo
Abstract：Objective To explore the predictive value of serum miR-181-5p,miR-21-3p,and miR-124-3p in sepsis patients complicated by acute respiratory distress syndrome(ARDS).Methods A total of 184 sepsis pa-tients admitted to Nanyang Central Hospital between March 2023 and March 2024 were selected.Based on whether ARDS occurred within 72 hours of hospital admission,the patients were divided into an ARDS group(60 patients)and a non-ARDS group(124 patients).Serum levels of miR-181-5p,miR-21-3p,and miR-124-3p were measured using real-time quantitative PCR(qRT-PCR).Receiver operating characteristic(ROC)curves were used to assess the pre-dictive value of these microRNAs for ARDS in sepsis patients.Multivariate logistic regression analysis(using the enter method to screen variables)was performed to analyze the factors influencing the occurrence of ARDS in sepsis patients.Results Compared with the non-ARDS group,the ARDS group had significantly higher APACHE Ⅱ scores(18.15±2.64 vs.12.05±2.41),CRP[(16.22±2.14)mg/L vs.(14.02±2.07)mg/L],and PCT[(3.84±0.58)ng/mL vs.(2.97±0.42)ng/mL](P＜0.05).The serum levels of miR-181-5p(0.85±0.14 vs.1.02±0.15),miR-21-3p(0.87±0.15 vs.1.03±0.14),and miR-124-3p(0.86±0.14 vs.1.01±0.12)were significantly lower in the ARDS group compared to the non-ARDS group(P＜0.05).The combination of serum miR-181-5 p,miR-21-3p,and miR-124-3p levels had a significantly higher area under the ROC curve(AUC)for predicting ARDS in sepsis patients than when each was assessed separately(Z=4.893,3.831,4.608,P＜0.001).Multivariate logistic regression analysis showed that APACHE Ⅱ score(OR=2.547),miR-181-5p(OR=0.625),miR-21-3p(OR=0.755),and miR-124-3p(OR=0.725)were significant factors influencing the occurrence of ARDS in sepsis patients(P＜0.05).Conclusion In sepsis patients complicated by ARDS,serum miR-181-5p,miR-21-3p,and miR-124-3p levels are downregulated.The combination of these three microRNAs has significant predictive value for the develop-ment of ARDS in sepsis patients.
Keywords：sepsisacute respiratory distress syndromemicroribonucleic acid-181-5pmicroribonucleic acid-21-3pmicroribonucleic acid-124-3pprediction
Publication Date：2025-04-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 565-569 )