Expression and clinical significance of LINC00152 and LINC00265 in serum of children with acute lymphoblastic leukemia
CHEN Xiao-cui
WANG Li-xue
WANG Wen-fang
CHEN Qiu-li
Abstract：Objective To investigate the expression levels of serum LINC00152 and LINC00265 in children with acute lymphoblastic leukemia(ALL)and their clinical significance.Methods A total of 108 children with ALL(ALL group)and 50 healthy children(control group)were enrolled prospectively.ALL patients were further divided into remis-sion(ni=65)and non-remission groups(n=43)based on the outcomes of induction remission chemotherapy.Serum levels of LINC00152 and LINC00265 were measured using real-time quantitative PCR.Receiver operating characteristic(ROC)curves were plotted to evaluate the diagnostic value of LINC00152 and LINC00265 for ALL.Kaplan-Meier sur-vival curves were drawn,and a multivariate Cox regression model was used to analyze risk factors affecting survival.Results Serum levels of LINC00152(2.53±0.72 vs.1.20±0.26)and LINC00265(1.74±0.45 vs.0.85±0.13)were significantly higher in the ALL group than in the control group(t=16.817,13.548,P＜0.001).Levels of LINC00152(2.95±0.84 vs.1.61±0.47)and LINC00265(1.92±0.53 vs.1.13±0.24)were significantly higher in the non-remission group than in the remission group(t=16.905,11.326,P＜0.001).ROC curve analysis revealed optimal cut-off values of 1.94 for LINC00152(AUC=0.882,95％ CI:0.819-0.943)and 1.31 for LINC00265(AUC=0.847,95％ CI:0.791-0.912).Higher expression of LINC00152 and LINC00265 was associated with in-creased rates of lymphadenopathy,hepatosplenomegaly,WBC count ≥50×109/L,platelet count＜100×109/L,high-risk stratification,mixed lineage leukemia(MLL)rearrangement,and chromosomal abnormalities(P＜0.05).Kaplan-Meier analysis indicated that high expression of LINC00152 and LINC00265 correlated with shorter survival(P＜0.05).Multivariate Cox regression analysis identified hepatosplenomegaly,high-risk stratification,MLL rearrangement,LINC00152≥1.94,and LINC00265 ≥1.31 as independent risk factors affecting survival.Conclusion LINC00152 and LINC00265 are highly expressed in the serum of children with ALL and are associated with poor prognosis.Their levels have diagnostic and prognostic value,offering potential guidance for clinical decision-making.
Keywords：acute lymphoblastic leukemiachildrenLINC00152LINC00265clinical value
Publication Date：2025-04-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 525-531 )