LncRNA DSCAM-AS1 regulates the miR-431-5p/PRDX1 signaling axis to influence the malignant progres-sion of hepatocellular carcinoma cells
CHEN Zhi-fei
SUN Wei-tao
YAN Ting-qi
SUN Jiang-jiang
SHI Yan-ke
YU Sheng-cai
HUANG Wei
Abstract：Objective To explore the effects of long non-coding RNA(LncRNA)DSCAM-AS1 on hepatocel-lular carcinoma(HCC)cell proliferation,apoptosis,migration,invasion,and epithelial-mesenchymal transition(EMT)through the miR-431-5p/peroxiredoxin 1(PRDX1)signaling axis.Methods Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression of LncRNA DSCAM-AS1 and miR-431-5p in the nor-mal human hepatocyte cell line(L02)and the human hepatocellular carcinoma cell line(Hep G2).Hep G2 cells were transfected with si-DSCAM-AS1+miR-431-5p inhibitor,si-DSCAM-AS1+inhibitor NC,si-DSCAM-AS1,and si-NC were designated as the si-DSCAM-AS1+miR-431-5 p inhibitor group,si-DSCAM-AS1+inhibitor NC group,si-DSCAM-AS1 group,and si-NC group,respectively.Untreated Hep G2 cells were considered the NC group.The interaction between LncRNA DSCAM-AS1,miR-431-5p,and PRDX1 was validated through dual-luciferase reporter assays.qRT-PCR was also used to assess LncRNA DSCAM-AS1 and miR-431-5p expression in Hep G2 cells.Cell proliferation was measured by MTT assay,apoptosis by flow cytometry,invasion and migration by Tr-answell assays,and protein expression(Ki-67,N-cadherin,Vimentin,E-cadherin,and PRDX1)by Western blot.Results Compared to L02 cells,Hep G2 cells exhibited significantly higher levels of LncRNA DSCAM-AS1 and PRDX1 expression(P＜0.05),whereas miR-431-5p levels were markedly reduced(P＜0.05).Compared to the NC and si-NC groups,the si-DSCAM-AS1 group demonstrated significantly reduced A570 absorbance,LncRNA DSCAM-AS1 ex-pression,invasive cell numbers,N-cadherin,migrating cell numbers,Vimentin protein expression,and Ki-67(P＜0.05).Additionally,the si-DSCAM-AS1 group showed significantly increased apoptosis rates,miR-431-5p expres-sion,and E-cadherin protein levels(P＜0.05).Notably,inhibiting miR-431-5p expression attenuated the suppres-sive effects of LncRNA DSCAM-AS1 silencing on Hep G2 cell proliferation,migration,and invasion.LncRNA DSCAM-AS1 was found to negatively regulate the miR-431-5p/PRDX 1 axis.Conclusion Silencing LncRNA DSCAM-AS1 may suppress the malignant progression of HCC by upregulating miR-431-5p,thereby downregulating PRDX1 expression.This modulation induces apoptosis,inhibits proliferation,and attenuates EMT in Hep G2 cells.
Keywords：LncRNA DSCAM-AS1miR-431-5p/PRDX1 axishepatocellular carcinomaproliferationap-optosisepithelial-mesenchymal transitionmigrationinvasion
Publication Date：2025-02-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 167-175 )