The expression and role of microRNA-143-3p in glioma

AN Zhan-sen; ZHENG Cui-hong; LIU Jing; HE Ze-feng; LIU Liang; LIU Ying-zi

doi:10.13820/j.cnki.gdyx.20242212

Abstract：Objective To examine the differential expression of microRNA-143-3p(miR-143-3p)in glio-mas and normal brain tissues,investigating its association with glioma clinicopathological features and its potential biologi-cal effects on glioma cell behavior.Methods Fifty-five glioma tissue samples and fifteen normal brain tissue samples from patients with traumatic brain injury or hemorrhage were collected from October 2021 to October 2023 at the Fourth Hospital of Hebei Medical University.Quantitative reverse transcription polymerase chain reaction(qRT-PCR)was used to determine miR-143-3p levels in glioma and normal brain tissues,as well as in human astrocytes and glioma cell lines(T98,U87,U251).The impact of miR-143-3p overexpression on glioma cell proliferation,invasion,migration,cell cycle progression,and apoptosis was evaluated using scratch assays,Transwell assays,CCK-8 assays,and flow cytome-try.Results miR-143-3p expression was significantly lower in glioma tissues compared to normal brain tissues(P＜0.000 1).Low miR-143-3p expression correlated with glioma grade(P＜0.001)but showed no significant association with age,gender,or KPS score(P＞0.05).Similarly,miR-143-3p expression in glioma cell lines(T98,U87,U251)was significantly lower than in human astrocytes(P＜0.01).Overexpression of miR-143-3p markedly inhibited glioma cell proliferation,migration,and invasion(P＜0.01),induced cell cycle arrest in the G0/G1 phase,decreased proliferation indices,and significantly increased apoptosis rates(P＜0.01).Conclusion miR-143-3p is downregu-lated in glioma tissues,and its reduced expression is associated with glioma malignancy.miR-143-3p inhibits glioma cell proliferation,invasion,and migration while promoting apoptosis,suggesting its potential as a therapeutic target in glioma.