Study on the mechanism of Zhitongshengji Ointment in promoting wound repair in rats with chronic refractory ulcers
LI Shun-ning
HU Liu-ping
ZHANG Jin-sheng
LI Jian-yi
Abstract：Objective To investigate the mechanism of Zhitongshengji Ointment in promoting the healing of chro-nic refractory ulcers in rats.Methods A total of 72 rats were used to establish a model of chronic refractory ulcers.They were randomly divided into two groups:the vaseline control group(control group)and the Zhitongshengji Ointment treat-ment group(observation group),with 36 rats in each group.The wounds were treated once daily,and wound healing was observed on days 3,7,and 14.Wound area and healing rate were calculated using a digital camera combined with ImageJ software.Histopathological changes in the ulcer wound tissue were observed by HE staining,and collagen protein expres-sion was detected by Masson staining combined with ImageJ software.Western blot(WB)analysis was used to measure the expression levels of basic fibroblast growth factor(bFGF),epidermal growth factor(EGF),and vascular endothelial growth factor(VEGF)proteins in the ulcer wound tissues.Results Compared with the vaseline control group,no signifi-cant difference was observed after 3 days of intervention.However,on day 7 and day 14,the wound area in the observa-tion group was significantly reduced,and the wound healing rate was significantly increased(P＜0.05).HE and Masson staining showed that,compared to the control group,the observation group had fewer inflammatory cells,more new capil-laries,collagen fibers,and fibroblasts,and significantly more granulation tissue and epithelial tissue formation.On day 3,the expression level of EGF protein in the wound tissues of the observation group was higher than that in the control group(P＜0.05).Although the expression levels of bFGF and VEGF proteins increased,the differences were not statistically significant compared with the control group(P＞0.05).As compared with day 3,the levels of EGF,bFGF,and VEGF in the wound tissues on day 7 and day 14 were significantly increased,with statistically significant differences(P＜0.01,P＜0.05).Conclusion Zhitongshengji Ointment can upregulate the expression of growth factors such as EGF,bFGF,and VEGF in ulcer tissues,promoting fibroblast proliferation,angiogenesis,and granulation growth in wound tissues.This may be the mechanism by which it promotes wound repair.
Keywords：Zhitongshengji Ointmentchronic refractory ulcerswound healinggrowth factorsrat
Publication Date：2024-11-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 1397-1403 )