Correlation of circulating exosomal miR-485-3p and miR-32-5p expression with the incidence of early-on-set coronary heart disease
TIAN Han-bing
LI Qing-yong
LI Pan-rong
SU Jin-ling
SUN Qi
LI Shao-jing
WANG Fan
Abstract：Objective To investigate the correlation between circulating exosomal miR-485-3p,miR-32-5p expression,and the incidence of early-onset coronary heart disease(CHD).Methods This study retrospectively in-cluded 50 patients with a confirmed diagnosis of early-onset CHD from October 2023 to May 2024 as the observation group and 50 matched,healthy individuals without CHD as the control group.Demographic and clinical data were collect-ed and compared.Multivariate logistic regression analysis was applied to evaluate the significant predictors of early-onset CHD.Additionally,Spearman correlation analysis was used to assess the relationship between plasma exosomal miR-485-3p,miR-32-5p levels,and CHD occurrence and severity,indicated by the Gensini score.The diagnostic utility of exosomal miR-485-3p and miR-32-5p,individually and combined,was assessed using receiver operating charac-teristics(ROC)curve analysis.Results The observation group exhibited significantly higher proportions of family history of CHD,smoking history,low density lipoprotein(LDL)levels,miR-485-3p,miR-32-5p,and Gensini scores than the control group(P＜0.05).Logistic regression analysis indicated that smoking history,family history of CHD,LDL,miR-485-3p,and miR-32-5p were key risk factors for early-onset CHD,the OR values were 6.997(95％CI:1.578-31.027),16.671(95％CI:1.666-166.799),2.632(95％CI:1.060-6.537),12.717(95％CI:2.629-61.505),6.000(95％CI:1.839-19.571).respectively.Positive correlations were found between early-onset CHD and elevated levels of circulating exosomal miR-485-3p and miR-32-5p(r=0.525 and 0.548,respectively;P＜0.05),as well as between the Gensini score and these two exosomal miRNAs(r=0.485 and 0.506,respectively;P＜0.05).The ROC curve analysis yielded the area under ROC curve(AUC)values of 0.927 and 0.936 for miR-485-3p and miR-32-5p,respectively,with a combined predictive AUC of 0.957.Conclusion Circulating exosomal miR-485-3p and miR-32-5p are upregulated in early-onset CHD,demonstrating positive correlations with both disease in-cidence and coronary lesion severity.These miRNAs may serve as predictive biomarkers for early-onset CHD,with high-er diagnostic accuracy when combined.
Keywords：early-onset coronary artery diseaseexosome miR-485-3pexosome miR-32-5pcorrelation
Publication Date：2024-11-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1374-1379 )