Clinical value of mNGS in pathogen diagnosis of elderly patients with community-acquired pneumonia
LI Xing-ming
XIE Xiu-fang
WANG Xian-gang
LI Yun
YUAN Xiang
Abstract：Objective To analyze the clinical value of metagenomic next-generation sequencing(mNGS)in the pathogen detection of elderly patients with community-acquired pneumonia(CAP).Methods A total of 112 elderly CAP patients were included in the study.The detection efficiency of pathogens using mNGS was compared with conven-tional microbiological tests(CMTs).Statistical analyses were conducted using Fisher's exact test and ROC curves to eval-uate the diagnostic differences between mNGS and CMTs.Results The overall diagnostic rate of mNGS was significantly higher than that of CMTs(94.6％vs.65.2％,P＜0.001).The detection time for mNGS was significantly shorter than that for CMTs[(26.3±5.9)h vs.(85.5±6.4)h;t=21.034,P＜0.05].mNGS detected a higher number of bacteri-al,viral,fungal,and atypical pathogens compared to CMTs.The detection rate for mixed infections with mNGS was 76.4％,significantly higher than CMTs'60.3％(x2=6.851,P＜0.05).Of the detected pathogens,there were 64 ca-ses of agreement,35 cases of partial agreement,and 13 cases of complete disagreement between mNGS and CMTs.Con-clusion mNGS demonstrates superior capability in detecting a broader spectrum of pathogens in elderly patients with com-munity-acquired pneumonia,highlighting its significant clinical value.
Keywords：community acquired pneumoniaelderlymetagenomic next-generation sequencingsconventional microbiological testsbronchoalveolar lavage fluid
Publication Date：2024-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1326-1330 )