Prognostic value of SNHG12 in bladder urothelial carcinoma

GU Zu-xuan; ZHOU Xiao-bing; YU Yang

doi:10.13820/j.cnki.gdyx.20233427

Abstract：Objective Long non-coding RNA(lncRNA)SNHG12 plays a crucial role in the proliferation and invasion of various malignancies.This study aims to assess the clinical significance of SNHG12 in the prognostic evaluation of bladder urothelial carcinoma(BUC).Methods The authors evaluated SNHG12 expression in 40 paired BUC tissues and adjacent non-cancerous tissues(ANCTs)using qRT-PCR.Univariate and multivariate analyses were performed in relation to the patients'clinical characteristics.Kaplan-Meier survival curves were constructed to study the difference in recurrence-free survival(RFS)between high and low expression groups,and ROC curves were used to analyze the prog-nostic value of SNHG12 in BUC.The differential expression of SNHG12 in BUC cells(5637)and normal bladder mucosal cells(SV-HUC-1)was also assessed by qPCR,and the effect of SNHG12 on cell proliferation,migration,and inva-sion was tested using siRNA transfection and plasmid overexpression in 5637 cells.Results SNHG12 expression was sig-nificantly elevated in BUC samples compared to ANCTs(P＜0.001).High SNHG12 expression was correlated with tumor size(P=0.01)and recurrence(P=0.003).Multivariate analysis indicated that SNHG12 is an independent prognostic factor for BUC.Patients with high SNHG12 expression had a shorter mean RFS compared to those with low SNHG12 ex-pression(P=0.016 7).The ROC analysis revealed that the AUC value of SNHG12 was 0.820 0(95％CI:0.718 2 to 0.921 8;P＜0.001).qPCR results showed a statistically significant difference in SNHG12 expression between 5637 and SV-HUC-1 cells,and CCK-8,Transwell,and Matrigel assays demonstrated that differential expression of SNHG12 affected the proliferation,migration,and invasion abilities of tumor cells.Conclusion These findings suggest that SNHG12 may be a unique and potential biomarker for the prognostic evaluation of BUC.