Bioinformatics analysis of upregulated COL7A1 in lung adenocarcinoma:prognosis and immune cell infiltration
YU Jing
ZHANG Chen-fei
Abstract：Objective To investigate the expression of COL7A1 in lung adenocarcinoma,its prognostic value,and its relationship with immune cell infiltration in the tumor microenvironment.Methods RNA-Seq data and clinical information from TCGA and GEO databases were analyzed to assess COL7A1 mRNA expression.Protein expression was e-valuated through proteomic analysis.The diagnostic value of COL7A1 in lung adenocarcinoma was assessed using ROC curves.Prognostic value was evaluated using Cox regression and Kaplan-Meier survival analyses,and a clinical predic-tion model was established.The TIME2.0 database was used to evaluate the correlation between COL7A1 and immune cell infiltration.RT-qPCR was performed to validate COL7 A1 expression.Results COL7A1 was significantly upregulated in lung adenocarcinoma tissues,particularly in those with distant metastasis and at advanced stages.COL7A1 showed diag-nostic and prognostic value in lung adenocarcinoma,with high COL7A1 expression being an independent risk factor.The constructed prediction model was more accurate than TNM staging.Additionally,COL7A1 was positively correlated with tumor fibroblast infiltration and negatively correlated with neutrophil infiltration.Conclusion High expression of COL7A1 in lung adenocarcinoma indicates poor prognosis and suggests that COL7A1 could be a potential target for immunotherapy.
Keywords：lung adenocarcinomaCOL7A1prognosisimmune cellsbioinformatics
Publication Date：2024-08-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 1053-1059 )