Validation of 6-Gingerol in inhibiting cartilage degeneration through USP49 via molecular docking technology
YANG Lan-bo
SU Chun-xia
ZHAO Xiao-fei
YANG Yan-mei
CHENG Yan
HUANG Tao
WANG Hai-bin
Abstract：Objective To understand the effects and underlying mechanisms of 6-gingerol on chondrocyte apop-tosis and cartilage degradation in osteoarthritis.Methods The active components of traditional Chinese medicine that sig-nificantly upregulate USP49 expression(6-gingerol)were screened and subjected to molecular docking.Subsequently,IL-1 β-induced chondrocytes were treated with different concentrations of 6-gingerol,and the apoptosis of chondrocytes and the expression of USP49,β-catenin,and cartilage degradation-related proteins(MMP-1,MMP-13)were de-tected.Results The screening revealed that 6-gingerol significantly promotes the expression of USP49.Molecular doc-king showed a high-efficiency binding between 6-gingerol and USP49.After treating IL-1β-induced chondrocytes with different concentrations of 6-gingerol,the expression of USP49 was significantly upregulated,and the apoptosis of chondrocytes and the expression of β-catenin,MMP-1,and MMP-13 were inhibited.Conclusion 6-gingerol regu-lates the Wnt/β-catenin signaling pathway by upregulating USP49 expression,inhibits chondrocyte apoptosis and the ex-pression of cartilage degradation-related proteins MMP-1 and MMP-13,thereby alleviating IL-1 β-induced cartilage degeneration.
Keywords：6-gingerolUbiquitin specific proteases 49Molecular dockingWnt/β-catenin pathway
Publication Date：2024-08-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 970-975 )