Silencing of Periostin gene modulates murine osteoblast metabolism to inhibit bone resorption activity
CAI Jun
QIN Han
Abstract：Objective To investigate the molecular mechanisms by which silencing of the Periostin gene affects the expression of Runx2,receptor activator of nuclear factor-kappa B ligand(RANKL),and osteoprotegerin(OPG)in murine osteoblasts and its impact on bone resorption activity.Methods Murine osteoblast cell line MC3T3-E1 was di-vided into an experimental group and a control group.The experimental group was transfected with LVpFU-GW-016PSC66473-1 virus,while the control group received pFU-GW-016PSC53349-1 virus to ensure equivalent cellu-lar conditions.The efficiency of gene silencing was assessed using MDC method,and the protein expression of Runx2,RANKL,and OPG in both groups was evaluated by Western blotting.Results Following Periostin silencing,the experi-mental group showed significantly enhanced fluorescence expression compared to the control group(P＜0.01).Protein expression of Runx2 and RANKL was significantly decreased(P＜0.05),whereas OPG expression was markedly in-creased(P＜0.05)in the experimental group compared to the control group.Conclusion Silencing of Periostin alters RANKL and OPG expression in murine osteoblasts,potentially influencing the Runx2 gene via the nuclear factor-kappa B(NF-κB)signaling pathway.This enhancement of osteoblast function may contribute to the inhibition of bone resorp-tion.
Keywords：PeriostinRunx2RANKLOPGosteoblastbone metabolism
Publication Date：2024-07-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 819-823 )