Expression significance of Myo,Gas6,and SOX9 mRNA in patients with acute myocardial infarction and the prognostic value of combined detection after percutaneous coronary intervention

GUO Hui-min; AN Jing-xia; LI Hui

doi:10.13820/j.cnki.gdyx.20231348

Abstract：Objective To investigate the expression significance of myoglobin(Myo),growth arrest-specific protein 6(Gas6),and SRY-related high-mobility group box 9(SOX9)in patients with acute myocardial infarction(AMI)and evaluate their combined detection value for prognosis assessment after percutaneous coronary intervention(PCI).Methods A total of 166 patients with AMI admitted between January 2020 and June 2021 were included in the study group,and 90 healthy volunteers were recruited as the control group.Serum levels of Myo and Gas6 were measured by enzyme-linked immunosorbent assay,and SOX9 mRNA was detected using the SOX9 mRNA isolation kit.Patients in the study group were divided into MACCE group and non-MACCE group based on the occurrence of major adverse cardio-vascular and cerebrovascular events(MACCE)during hospitalization.The expression of Myo,Gas6,and SOX9 mRNA was compared between the study and control groups,as well as before and after PCI in AMI patients.Clinical differences between the MACCE and non-MACCE groups were analyzed using univariate/multivariate analysis.The prognostic value of combined Myo,Gas6,and SOX9 mRNA detection for AMI prognosis was assessed.Results Compared with the con-trol group,the study group showed significantly higher levels of Myo and Gas6,and lower expression of SOX9 mRNA(P＜0.05).After PCI,patients in the study group exhibited significantly decreased expression of Myo and Gas6,and in-creased expression of SOX9 mRNA(P＜0.05).The MACCE group had significantly higher levels of Myo and Gas6 and lower expression of SOX9 mRNA compared to the non-MACCE group(P＜0.05).Logistic regression analysis showed that age,postoperative bleeding,Myo,Gas6,and SOX9 mRNA were independent risk factors affecting the occurrence of MACCE in AMI patients treated with PCI(P＜0.05).The area under the ROC curve for combined assessment of AMI prognosis using Myo,Gas6,and SOX9 mRNA was 0.936,with higher specificity and sensitivity compared to individual detection of Myo,Gas6,and SOX9 mRNA(0.706,0.696,and 0.711,respectively;Z=2.261,P＜0.05).Conclu-sion For patients with AMI,higher expression of Myo and Gas6 and lower expression of SOX9 mRNA were observed,and PCI treatment effectively improved the levels of Myo,Gas6,and SOX9 mRNA.Combined detection of these three bio-markers has a significant predictive role for MACCE after PCI,facilitating early diagnosis and treatment decision-making for patients,and warrants clinical application.