The correlation between the expression of CD44 and TLR4 in the ear effusion of children with secretory otitis media and adenoid hypertrophy

ZHAO Yan-yan; ZHANG Su-na; HU Hao-lei; ZHANG Peng-zhen; ZHAO Jia; LI Shuo; LI Yi

doi:10.13820/j.cnki.gdyx.20231973

Abstract：Objective To detect the expression levels of CD44 and TLR4 in the ear effusion of children with se-cretory otitis media(SOM)and analyze their correlation with adenoid hypertrophy.Methods Eighty children diagnosed with secretory otitis media were selected as the observation group,and 100 healthy children undergoing routine health ex-aminations during the same period were chosen as the control group.The expression levels of CD44 and TLR4 in the ear effusion of the observation group and peripheral blood of both groups were measured.The correlation between CD44 and TLR4 expression levels and adenoid hypertrophy was analyzed using Pearson's method.The diagnostic value of CD44 and TLR4 for adenoid hypertrophy was assessed using receiver operating characteristic(ROC)curve analysis.Results The expression levels of CD44 and TLR4 in the peripheral blood and ear effusion of children in the observation group were sig-nificantly higher than those in the peripheral blood of the control group(P＜0.05).The expression levels of CD44 and TLR4 in the ear effusion of the observation group were significantly higher than those in the peripheral blood of the same group(P＜0.05).In the observation group,the expression levels of CD44 and TLR4 in serous ear effusion were signifi-cantly lower than those in mucous ear effusion(P＜0.05).Additionally,the expression levels of CD44 and TLR4 in the ear effusion of children with chronic otitis media were significantly lower than those in children with acute otitis media(P＜0.05).Furthermore,the expression levels of CD44 and TLR4 in the ear effusion of children with adenoid hypertro-phy and otitis media were significantly higher than those in children with normal adenoids and otitis media(P＜0.05).There was a positive correlation between CD44 and TLR4 expression in the ear effusion of children with secretory otitis media(r=0.516,P=0.000).The diagnostic value of CD44 and TLR4 combined in diagnosing adenoid hypertrophy was significantly higher than that of either alone.Conclusion CD44 and TLR4 are upregulated in the ear effusion of children with secretory otitis media,and their expression levels are significantly increased in children with adenoid hypertrophy compared to those with normal adenoids,indicating a close association with adenoid hypertrophy.