Correlation between gut microbiota composition and metabolic functions in obese and non-obese patients with polycystic ovary syndrome
XU La-mei
GAO Jie
LI Ying
LIU Li-dong
Abstract：Objective To compare the characteristics of gut microbiota and metabolic functions in patients with polycystic ovary syndrome(PCOS),specifically focusing on the differences between obese and non-obese individuals.Methods Sixteen obese PCOS females(BMI ≥28 kg/m2),twelve non-obese PCOS females(BMI 18.5-23.9 kg/m2),and fourteen healthy young females(BMI 18.5-23.9 kg/m2)were selected from June 2020 to June 2022 as the non-obese normal group.Additionally,six healthy but obese females(BMI ≥28 kg/m2)were included as the obese normal group.Blood and non-menstrual fecal samples were collected and analyzed from all participants.Fecal samples were sequenced using 16S rDNA gene sequencing.Plasma metabolomics analysis was performed using liquid chromatogra-phy-tandem mass spectrometry.Results Compared to the non-obese normal group,non-obese PCOS showed signifi-cant increases in hirsutism scores,acne scores,LH,LH/FSH,testosterone,E2,and dehydroepiandrosterone(P＜0.05).In comparison to the obese normal group,obese PCOS exhibited significantly higher hirsutism scores,LH/FSH,and testosterone,along with significantly lower FSH(P＜0.05).Differential abundance analysis identified four genera(Finegoldia,Anaerococcus,Sneathia,and Adlercreutzia)significantly associated with obesity status(P＜0.05).Fine-goldia,Anaerococcus,Sneathia,and Adlercreutzia showed significantly lower abundance in obese females compared to non-obese females(P＜0.05).Stratified analysis based on PCOS status revealed a significant negative correlation between Finegoldia and Anaerococcus with obesity in both PCOS and non-PCOS females.There were suggestive but not significant interactions between PCOS and obesity with Sneathia and Adlercreutzia(PPCOS×Obesity=0.090,0.057).Anaerococcus ex-hibited a negative correlation with the ratio of indole-3-acetic acid to tryptophan and valine(r=-0.38,-0.30,P=0.007,0.041),while Adlercreutzia showed a negative correlation with proline and alanine(r=-0.37,-0.32,P=0.012,0.034).Conclusion In PCOS females,obesity is associated with changes in gut microbiota and plasma metabo-lites,including the depletion of bacteria producing butyrate and increased tryptophan degradation metabolism.These find-ings suggest potential interrelationships among gut microbiota,obesity,and PCOS.
Keywords：obesitypolycystic ovary syndromegut microbiomemetabolic function
Publication Date：2024-02-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 145-151 )