Expression of BAIAP2L2 in hepatocellular carcinoma and its prognostic significance
YUAN Ke-rong
GE Ying-nan
PAN Hong-yan
GONG Da
MO Jia-ye
DENG Xue-song
Abstract：Objective To analyze the expression pattern and prognostic relationship of brain-specific angiogene-sis inhibitor 1-associated protein 2-like 2(BAIAP2L2)in hepatocellular carcinoma(LIHC)from a bioinformatics per-spective,explore its potential mechanisms,and investigate its correlation with clinical characteristics in LIHC clinical ca-ses.Methods Analyze the differential expression of BAIAP2L2 in pan-cancer tissues and normal tissues using TCGA,GEPIA,GEO,and TIMER databases.Perform univariate and multivariate regression analysis to assess the relationship be-tween BAIAP2L2 expression and prognosis.Use Spearman analysis to evaluate the correlation between BAIAP2L2 expres-sion and genomic instability.Employ ssGSEA algorithm and KEGG enrichment analysis to investigate signaling pathways associated with BAIAP2L2 expression.In clinical cases,assess BAIAP2L2 expression in 55 LIHC tissues,adjacent tis-sues,and 10 normal liver tissues using immunohistochemistry,and analyze its correlation with pathological features and prognosis.Results BAIAP2L2 was universally highly expressed in pan-cancer tissues,showing differences compared to corresponding adjacent tissues and normal tissues.Its overexpression was significantly associated with poor prognosis in seven cancers,including adrenocortical carcinoma(ACC),low-grade glioma(LGG),mesothelioma(MESO),prostate adenocarcinoma(PRAD),skin cutaneous melanoma(SKCM),uterine corpus endometrial carcinoma(UCEC),and uve-al melanoma(UVM).The expression level of BAIAP2L2 was positively correlated with tumor mutation burden(TMB)in esophageal carcinoma(ESCA),pancreatic adenocarcinoma(PAAD),and UCEC(P＜0.05).It was positively correla-ted with microsatellite instability(MSI)in cholangiocarcinoma(CHOL),diffuse large B-cell lymphoma(DLBC),UVM,and LGG,while negatively correlated with MSI in colorectal adenocarcinoma(READ)(P＜0.05).BAIAP2L2 participated in the regulation of 16 signaling pathways in LIHC,positively correlating with pathways such as glycosphingo-lipid biosynthesis/neolacto synthesis,G2/M checkpoint,tumor proliferation,sphingolipid metabolism,and N-glycan bi-osynthesis.Conversely,it negatively correlated with pathways like fatty acid degradation,primary bile acid biosynthesis,beta-alanine metabolism,biotin metabolism,butanoate metabolism,lysine degradation,D-arginine/D-ornithine me-tabolism,selenium compound metabolism,degradation of valine/leucine/isoleucine,tryptophan metabolism,and caffeine metabolism.Clinical data revealed that BAIAP2L2 expression in LIHC tissues was significantly higher than in adjacent tis-sues(P＜0.000 1)and normal liver tissues(P=0.003 6).It correlated with tumor number(P=0.038),diameter(P=0.010 1),and differentiation degree(P=0.031 4).The postoperative overall survival of the BAIAP2L2 high-ex-pression group was lower than that of the low-expression group(P=0.018 2).Univariate analysis indicated that the ex-pression level of BAIAP2L2 was associated with the survival prognosis of LIHC patients.Conclusion BAIAP2L2 promotes the occurrence and development of tumors by regulating TMB,MSI,and multiple signaling pathways.BAIAP2L2 is signifi-cantly overexpressed in LIHC compared to corresponding adjacent tissues and normal liver tissues,and it is closely related to tumor number,diameter,and differentiation degree,serving as an important factor in promoting tumor progression and cau-sing adverse prognosis.
Keywords：BAIAP2L2bioinformaticsliver hepatocellular carcinomaprognosisclinical features
Publication Date：2024-01-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：11( 83-93 )