Differential proteomic study of ovarian reserve decline and premature ovarian insufficiency model mice

LI Xiao-rong; ZHONG Jia-wen; QIN Ling; GAO Ting; LUO Yu-xue; MA Xiao-hong

doi:10.13820/j.cnki.gdyx.20231870

Abstract：Objective To explore the protein differences between decreased ovarian reserve(DOR),premature ovarian failure(POF),and the normal control group using proteomic technology;and Additionally,to analyze the differ-ential proteins to further elucidate the possible mechanisms of ovarian reserve decline and premature ovarian failure.Methods DOR model mice were prepared by a single intraperitoneal injection of 60 mg/kg cyclophosphamide combined with 6 mg/kg busulfan,while POF model mice were prepared by a single intraperitoneal injection of 120 mg/kg cyclophos-phamide combined with 12 mg/kg busulfan.The mice's estrous cycles were continuously observed after drug administra-tion to determine the success of the modeling.After successful modeling,differential proteins were screened,and bioinfor-matics methods were used for functional annotation and enrichment analysis of the identified differential proteins to analyze their possible mechanisms.Results A total of 148 differential proteins with fold changes of more than 1.2 were screened in the DOR group,and 149 differential proteins were identified in the POF group.Enrichment analysis showed that the differentially expressed proteins in the DOR group were mainly involved in biological processes such as vitamin B6 metabo-lism,pyridoxal phosphate synthesis,and oxidative stress,and they were enriched in pathways such as vitamin B6 metabo-lism,α-linolenic acid metabolism,and PPAR signaling.On the other hand,the differentially expressed proteins in the POF group were mainly involved in biological processes such as lipid metabolism and inflammatory response,and they were enriched in pathways such as glycosphingolipid biosynthesis,arachidonic acid metabolism,and ribosome.Conclu-sion The pathogenesis of DOR and POF is complex,involving processes like oxidative stress and inflammatory response.The α-linolenic acid signaling pathway may be an important pathway involved in the occurrence of DOR,and HIST1H1C,CA3,and FABP4 may be important targets for its occurrence.The arachidonic acid metabolism signaling pathway may be an important pathway involved in the occurrence of POF,and HIST1H1C,D2HGDH,and CYP4F3 may be important targets for its occurrence.