The effect of cell administration routes on bone marrow mesenchymal stem cell distribution in organ transplant recipients

GONG Yong-quan; CAO Jian-bin; WEI Cheng-xin; LI Fu-li; HUANG Wei-jia; HE Ling-yun; NONG Wei; WU Yi-wen; LUO Peng

doi:10.13820/j.cnki.gdyx.20183716

Abstract：Objective To investigate bone marrow mesenchymal stem cells distribution in organ transplant recipient with different cell administration routes. Methods Bone marrow mesenchymal stem cells (BMSCs) with enhanced green fluorescent protein (GFP) expression were isolated from transgenic mice. Cervical heart transplantation were established using C57 mice as both recipient and donor, which were randomly divided into two groups. In Group A, all the recipients were injected with 30 μL cell suspension containing 1*106 BMSCs through heart graft ascending aorta immediately after heart transplantation. In Group B, the same dose cell suspension were injected through heart graft right ventricle cavity at the same time point. The animals were sacrificed on Day 1, 3, 7, and 14 after surgery; and the liver, lung and heart graft were harvested for cells survival detection through histological examination. Results Twenty-four cases of cervical heart transplantation were performed without death. All the animals tolerated well after stem cells injection. In Group A, the eGFP+ cells were detected in heart graft and lung except for the liver on Day 1. More eGFP+ cells were revealed in Group A than Group B in the heart grafts, while with less eGFP+ cells in the lungsm on Day 1. On Day 3, the eGFP+ cells in both group were reduced compared to Day 1 in the heart grafts and lungs. More eGFP+ cells were revealed in Group A than Group B in the heart grafts, while less eGFP+ cells in the lungs on Day 3. And On Day 3, eGFP+ cells were detected in the livers. On Day 7, no eGFP+ cell was detected in the heart grafts, lungs and the livers in both group, except for the heart grafts in Group A. On Day 14, no eGFP+ cell was detected in all the tissues in both group. Conclusion Administration of BMSCs through graft arterial injection can lead to more cells accumulation in the transplanted organs and longer cells survival compare to venous injection. More cells are trapped in the lung after venous injection.