Expression of peroxisome proliferators-activated receptor-α and oxidative stress in normal liver tissues after TAE for hepatocellular carcinoma

LI Juan-juan; DU Wei; GUO Wei-lian; WANG Guang-ming

doi:10.13820/j.cnki.gdyx.20182413

Abstract：Objective To investigate the roles of peroxisome proliferators-activated receptor-α (PPAR-α) and oxidative stress (OS) in hepatocyte injury after hepatic carcinoma transarterial catheter embolization (TAE). Methods Twenty-seven New Zealand white rabbit VX2 hepatocellular carcinoma (HCC) models were successfully established. They were randomly divided into three groups, Group Control, Group Contrast/Angiopgraphy and Group TAE. The rabbits in Group Angiography or TAE were injected with contrast agent or treated with TAE, respectively. The rabbits in the Group TAE and Group Angiography were sacrificed 10 hours after TAE treatment and contrast injection. The activity or content of oxidative stress indicators (SOD, CAT, GSH-Px and MDA) on paracancerous liver tissues were detected in all the groups by biochemical enzymatic assay. Immunohistochemical analysis was used to detect the expression of PPAR-α in liver tissues.Results Compared with the Group Control, no significant difference was revealed in the activity of antioxidant indexes SOD, CAT and GSH-Px in liver tissue or the content of MDA peroxidation product in Group Angiography (P＞0.05). Compared with the Group Angiography, the anti-oxidation indexes of SOD, CAT and GSH-Px in the liver tissue of the Group TAE group were significantly reduced; while the MDA content of the peroxidation product was significantly increased (P＜0.01). Compared with the Group Control, the expression of PPAR-hein Group Angiography showed decreasing trend, but the difference was not statistically significant (P＞0.05). Compared with the Group TAE, the expression of PPAR-atistica Group Angiography showed significant increased trend (P＜0.01). Conclusion In hepatocellular carcinoma after TAE, the expression of PPAR-α is reduced in paracancerous liver tissues. The activities of antioxidant indexes like SOD, CAT and GSH-Px are reduced, the content of MDA in peroxidation products is increased, and results in hepatocyte injury.