Differential expression of long non-coding RNA regulates hypoxia-induced epithelial-mesenchymal transition in glioma cells
TANG Shang-hong
SONG Lei
XIE Shu-le
LI Qun-xing
LIN Zhao-yu
Abstract：Objective To investigate the correlation between hypoxia-induced epithelial-mesenchymal transition associated long non-coding RNA in glioma cells and its capacities of invasion and metastasis. Methods The expression of long non-coding RNA in glioma cells was detected by microarray. The accuracy of the results of microarray and the expression of epithelial-mesenchymal transition related genes were verified by qRT-PCR. The expression of proteins was detected by Western blotting. The invasion and migration capacities were assessed by invasion and migration assays. Results Hypoxia induced epithelial-mesenchymal transition of glioma cells. A number of differentially expressed long non-coding RNAs were found and long non-coding RNA HOTTIP was found up-regulated after hypoxia induction (P＜0.01). Reducing its expression level reversed the process of epithelial mesenchymal transition, and significantly reduced the invasion and migration capacities (P＜0.01). Conclusion The increased expression of long non-coding RNA HOTTIP may be closely related to hypoxia-induced epithelial-mesenchymal transition and enhanced invasion and migration.
Keywords：hypoxia-inducedepithelial-mesenchymal transitionlong non-coding RNAinvasion and migration
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 477-482 )