Protective effects of methane saline on intestinal injury in septic rats

LUO De-qiang; CHEN Zi-li; DAI Wei; GU Lin-ming

doi:10.13820/j.cnki.gdyx.20182084

Abstract：Objective To investigate the role of HO-1 on methane saline treatment for intestinal injury caused by sepsis and to explore its possible mechanism. Methods A total of 128 male SD rats (weighing 220-260 g and 8-10 weeks old) were randomly divided into 4 groups (n=32), Group Sham, Group Sepsis, Group Sham+MS and Group Sepsis+MS. Sepsis model was established by cecal ligation and puncture (CLP). Half hour and 12 h after CLP, rats in Group Sham+MS and Sepsis+MS were intraperitoneally injected with 10％ methane saline (10 mL/kg). Twenty rats in each group were used to observe the survival rate within 7 days after operation. CLP rats were sacrificed 6 h and 18 h after operation, and the jejunum tissues were collected for evaluation of histolopathological Chiu′s score and intestinal permeability. The peroxidation of superoxide dismutase (SOD), myeloperoxidase (MPO), tumor necrosis factor alpha (TNF-α) and interleukin-10 (IL-10) in the intestinal mucosa were detected by Elisa. The expression of Nrf2, HO-1 and tight junction protein (Occludin) were assessed by Western blot.Results Compared with Group Sham, the Chiu′s scores were significantly increased, while the expression of Nrf2, HO-1 and Occludin proteins were significantly up-regulated in Group Sepsis and Sepsis+MS (P＜0.05). Compared with Group Sepsis, Chiu′s score in Group Sepsis+MS was significantly decreased, but the expression of Nrf2, HO-1 and Occludin proteins were significantly up-regulated (P＜0.05).Conclusion The mechanism of methane saline in reducing intestinal tissue injury in rats with sepsis is correlated to the activation of Nrf2/ARE pathway and the up-regulation of HO-1, thus to promote intestinal Occludin expression.