The effects of Ureaplasma urealyticum on the level of DCXR expression and apoptosis in human spermatozoa
YUE Yin-quan
MA Xiao-ping
GAO Xiao-qin
Abstract：Objective To study the effects of Ureaplasma urealyticum (Uu) on the level of DCXR expression and apoptosis in human spermatozoa. Methods According to semen analysis referred to WHO standard, 145 cases were assigned in infertile group. Western blot was used to measure the level of DCXR expression on spermatozoa. The localization of this protein on human spermatozoa was determined by indirect immunofluorescent staining using anti-DCXR antibody. The sperm apoptosis was assayed with TUNEL. After the treatment of sensitive antibiotics, the changes of indicators before and after treatment for Uu infection were compared. Results The level of DCXR protein expression and the DCXR-positive rate in Uu positive group were lower than those in Uu negative group. After treatment, the level of DCXR protein expression and the DCXR-positive rate were significantly higher than before therapy in Uu positive group (P＜0.05). The sperm apoptosis rate in Uu positive group was significantly higher than Uu negative group; after treatment, the sperm apoptosis rate was significantly reduced in Uu positive group (P＜0.05). Conclusion Uu infection may affect the level of DCXR protein expression and sperm apoptosis that influence fertility. After effective treatment of Uu infection, the sperm protein DCXR expression and sperm apoptosis would be obviously improved.
Keywords：spermUreaplasma urealyticumDCXRacrosinmale infertility
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 201-204 )