High-throughput sequencing of intestinal flora of rats with ovarian hyperstimulation syndrome
MO Yi
LI Yun-juan
NIU Xiang-li
XU Yi-chen
GONG Fang-qiang
WANG Juan
XIE Dan-ni
YUE Gui-hua
LIANG Fang-fang
Abstract：Objective To compare the characteristics of intestinal flora among rats with ovarian hyperstimulation syndrome and super stimulate ovulation, and normal rats, thus to investigate the effect of exogenous hormone on intestinal flora. Methods The ovarian hyperstimulation syndrome and the super stimulate ovulation rat models were constructed through subcutaneous injection of different doses of the hormone. The intestinal contents were collected, and bacterial whole DNA were extracted. The regions V3-V4 of 16 s rDNA gene were sequenced by high-throughput sequencing.Results The high-throughput sequencing coverage index of the intestinal contents of the three groups were all greater than 0.993. With the increase of exogenous hormone, the diversity of intestinal microflora was also increased. The predominant 3 phyla in these three groups were Firmicutes, Verrucomicrobia and Bacteroidetes. On the genus level, the predominant 4 genus were Lactobacillus, Akkermansia, Enterococcus and Clostridium XlVa. With the increase of exogenous hormones, the relative abundance of Akkermansia was decreased, while Enterococcus and Clostridium XlVa were increased. Conclusion The exogenous hormones injection alters the homeostasis and increase the diversity of intestinal flora in rats. It reduces the relative abundance of specific probiotics, while increases the relative abundance of pathogenic bacteria, resulting in corresponding overstimulation response.
Keywords：intestinal floraovarian hyperstimulation syndromeexogenous hormoneshigh throughput sequencingflora structure
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 172-175 )