Application of infrared thermal imaging in the rabbit femoral vein complete embolization model simulated by ligation

DENG Fang-ge; GUO Hui-zhi; LIN Lin; LI Tao

doi:10.13820/j.cnki.gdyx.20181350

Abstract：Objective To investigate the application of the infrared thermal imaging(IRTI)in the model of complete vein embolization caused by femoral vein ligation in New Zealand white rabbits. Methods The proposed model in this study is established by classical extracorporeal ligation performed on right femora vein, and the left served as sham operation controlling side. Peripheral blood was collected before operation, and 2 hours, 12 hours and 48 hours after operation. The inflammatory factors(IL-6, IL-8 and TNF-α)were detected by ELISA. IRTI were performed at T1(preoperative base point), T2(the moment after operation)and T3(48 hours after operation). The rabbits were sacrificed at T3, and bilateral femoral veins were observed and taken for immunohistochemical detection of NF-κB. Results The ligation side of all experimental rabbits had slightly swollen and limited activity. There were no statistical differences in peripheral blood inflammatory factors among different time points, but there was a positive expression of inflammatory factor NF-κB in the experimental femoral vein detected by immunohistochemical examination. The IRTI presented a relative symmetric distribution on both sides at T1. At T2, the body surface temperature of both sides was lower than that at T1, with asymmetry distribution that higher temperature on ligated side. The infrared thermal imaging trajectory at T3 were similar to that of T2, while the temperature of the control side was recovered to the level of temperature of T1. Conclusion Classical femoral vein embolism caused by ligatures in rabbits does not lead to the increase of inflammatory factors in peripheral blood, but ligated femoral vein has the inflammatory response. IRTI detection has a typical IRTI trajectory characteristics, which could be used for early diagnosis of DVT.