The expression and significance of Fas and FasL in the sperm of infertile patients
WANG Shu-yuan
HUANG Hui-lun
CHEN Rui-fang
LIU Hong-can
KE Pei-feng
SHI Han-zhen
Abstract：Objective To compare the difference in semen Fas/FasL gene expression of infertile patients and nor-mal matched participants ; and to investigate the correlations among the expression of Fas /FasL gene, main parameters of semen routine analysis and the male infertility.Methods Semen samples of 60 cases diagnosed as infertility patients (infertility time more than one year ) and 60 cases from fertility function examination (planned to have the second child ) were collected from Guangdong Provincial Hospital of Chinese Medicine and Haizhu Maternal and Child Health Hospital . Spanish SCA were used to detect the main parameters of semen (including the sperm density , total sperm activity rate, sperm progressive motility rate and total progressive sperm count ).Fas and FasL expression was measured by quantitative real－time PCR.Results The semen volume and total rate of sperm activity , sperm progressive motility and total pro-gressive sperm count of the infertility patients were significantly reduced , while the Fas/FasL gene expression was signifi-cantly increase.Conclusion The up－regulation of Fas/FasL genes is correlated to the main parameters of sperm , which may be related factors to incomplete spermatogenesis dysfunction as well as male infertility .
Keywords：infertilityFas geneFasL genesquantitative real －time PCR
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1827-1829 )