The expression of Chemerin in cartilage and synovial fluid of patients with knee osteoarthritis
YANG Xi-ren
DU Gang
LI Wei
WEI Hua-liang
BAI Bing
LAO Shan
Abstract：Objective To study the roles of Chemerin in the pathogenesis of osteoarthritis (OA) via investigating the Chemerin expression in synovial fluid (SF) and cartilage.Methods Knee SF and cartilages of patients with knee OA were collected as experimental group , and normal cartilage of patients with femoral neck fracture or amputation were collected control group.Quantitative real－time polymerase chain reaction (qRT－PCR) and Western－blot ( Wb) were used to assess the expression of Chemerin/chemeR23 and PPARγ2 in cartilage, as enzyme－linked immunosorbent assay (Elisa) was used to assess the Chemerin concentration in SF.Results The Chemerin concentration in SF of patients with Kellgren－Lawrence 4 (K－L4) OA was significantly higher than those of patients with K －L2 and K－L3 OA, and there was a positive liner correlation between Chemerin and K －L grading of OA.QRT－PCR and Wb results demonstrated that the expression of Chemerin and PPAR γ2 in OA cartilage was significantly higher than that in control group , and there was a positive correlation between Chemerin and PPAR γ2.Conclusion The concentration of Chemerin in SF increases with the degree of knee OA according to K －L grading.The expression levels of chemerin and PPARγ2 in OA cartilage are higher than the control , and there is a positive liner relationship between Chemerin and PPAR γ2.Chemerin may play an important role in the occurrence and development of knee OA.The Chemerin concentration in SF may be a new index for the degree of OA.
Keywords：chemerincartilageSFosteoarthritisPPARγ2
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1806-1809 )