The effect of Buzhongyiqitang on the TAM drug resistance of metastatic breast cancer through SHP－1

WU Yi-fen; WANG Gang; TAN Qing-lin; YE Zhi-bin; PENG Shu-dao; WU Hong-yuan; YE Zhao-kang; LI Hao; ZHANG Han-wen

doi:10.3969/j.issn.1001-9448.2018.12.007

Abstract：Objective To study the effects of Buzhongyiqitang on the TAM drug resistance of metastatic breast cancer through SHP－1.Methods That TAM was added in culture medium for more than six months to induce drug －resistant breast cancer cell model MCF－7/TAM－R.The experiment groups were divided into MCF－7/TAM－RNC, MCF－7/TAM－RSHP－1 Ri, BZYQT+MCF－7/TAM－RNCand BZYQT +MCF－7/TAM－RSHP－1 Ri.SHP－1 was inhib-ited by RNA interference in breast cancer cell line MCF－7/TAM－RSHP－1 Ri.And MCF－7/TAM－RNCwas applied as negative control cell line.The two cell lines were inoculated in nude mice to establish the model of lung metastasis of breast cancer, and Buzhongyiqitang treatment was given.The tumor growth in nude mice was recorded.SHP－1, EGFR and HER－2 protein and mRNA expression were assessed by western －blot and qPCR, respectively.Results Compared with MCF－7/TAM－RNCgroup, the gene expression and protein expression of SHP－1 were increased; the gene expres-sion and protein expression of EGFR decreased ; the protein expression of HER －2 decreased; and the tumor size was smaller in BZYQT+MCF－7/TAM－RNCgroup.Compared with MCF－7/TAM－RNCgroup, the gene expression and protein expression of EGFR increased ; the protein expression of HER－2 increased; and the tumor size was bigger in MCF－7/TAM－RSHP－1 Rigroup.Compared with MCF－7/TAM－RNCgroup, the tumor growth was partially suppressed , the protein expression of EGFR and HER－2 increased, and the gene expression of EGFR decreased in BZYQT+MCF－7/TAM－RSHP－1 Rigroup.Conclusion Buzhongyiqitang inhibits the gene expression and protein expression of EGFR , and the protein expression of HER－2 by up－regulating the expression of SHP －1, and thus further reverses the TAM drug resistance of the MCF－7 cell and inhibits the tumor growth.