Effect of Physcion on proliferation and apoptosis of Rat Hepatic Stellate Cells
PAN Ting
LIU Yuan
Lü Zhi-ping
Abstract：Objective To study the effect of physcion on proliferation and apoptosis of rat hepatic stellate cells (HSC－T6 cell).Methods Physcion was prepared from Polygonum cuspidatum by solvent system separation method. CCK－8 assay was used to test the cell viability.Edu stain were used to assess proliferation of HSC －T6 cells.Western Blot assay was used to assess the expression of Bcl －2 and Bax.Results Compared with control group , physcion could inhibit the proliferation of HSC －T6 cells in a concentration －dependent manner.The number and rate of edu positive cells were significantly less in physcion group than those in control group (P＜0.001).Western Blot showed that the ex-pression of Bcl－2 was significantly reduced by physcion at 10ed t/L and 20ion /L; whereas Bax was significantly in-creased.Conclusion Physcion inhibits cell proliferation of HSC －T6, which may via adjusting Bax/Bcl－2 levels.
Keywords：Polygonum cuspidatumhepatic fibrosisphyscionHSC－T6 cellcell apoptosis
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1772-1775 )