Effects of propofol on invasion, metastasis and epithelial-mesenchymal transition of human hepatocellular carci-noma cells
PAN Shi-rui
HUANG Huan-sen
LU Guo-an
RUAN Lin
TAN Wan-lin
Abstract：Objective To investigate the influence of propofol on invasion , metastasis and epithelial -mesenchy-mal transition (EMT) of human hepatocellular carcinoma cells .Methods Hepatocellular carcinoma cells ( HCCLM3, QGY-7703, SMMC-7721) were stimulated with propofol in gradually increasing dosages (5 μg/mL, 10 μg/mL and 20 μg/mL).The effects of propofol on the migration and invasion of hepatocellular carcinoma cells were assessed by wound healing assay, trans-well matrix invasion assay and three -dimensional spheroid invasion assay .HCCLM3 cells were stimulated with propofol of 20 μg/mL.Western blot and immunofluorescence assay were used to detect the expression of EMT makers (E-cadherin and Vimentin ).Results With the increase of propofol concentration , wound healing assay showed that the migration ability of hepatocellular carcinoma cells significantly decreased ( P＜0.05); trans-well inva-sion experiments and three -dimensional culture experiments showed that the invasive ability of hepatocellular carcinoma cells significantly decreased (P＜0.05).The expression of E-cadherin was up-regulated and the expression of vimen-tin was down-regulated in HCCLM3 cells after propofol stimulation of 20 μg/mL (P＜0.05).Conclusion In vitro, propofol can suppress the migration and invasion of HCC cells with concentration -dependent effect , which may be corre-lated to the inhibition of EMT.
Keywords：propofolhepatocellular carcinomainvasion and metastasisepithelial-mesenchymal transition
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1449-1453 )