The correlation analysis between abnormal lipid metabolism and FABP4 expression in placenta tissue of rats with hypertension of pregnancy
GAO Ting-ting
SONG Yan-chun
WANG Yan-hua
ZHANG Hui
HUI Shu-ning
MA Yan-qiong
MA Xiao-li
WU Kai
ZHANG Ming-hao
XU Hua
ZHANG Hui-ping
Abstract：Objective To investigate the correlation between placenta tissue lipid metabolic disorder and lard type fatty acid binding protein 4 (FABP4) and its role in hypertension of pregnancy .Methods Twenty female SD rats (weight range from 250-300 g, SPF grade) were randomly divided into 2 groups (n＝10 for each group), control group (Group PC) and hypertension of pregnancy group (Group HDCP).The rats in the 2 groups were injected with normal sa-line and nitric oxide synthase inhibitor (L-NAME) 50 mg/( kg· d) to induced hypertension of pregnancy model (HD-CP), respectively.Blood pressure and urine protein were recorded .Placental tissues were collected for HE staining .En-zymatic method was used to assess the total cholesterol (TC), free cholesterol (FC), cholesterol ester (CE), free fatty acid (FFA) and triglyceride (TG) content in placental tissues .qRT-PCR and Western blot were used to detected the expression of FABP4 in placental tissue.The correlation between the expression of FABP 4 protein and the contents of TC and TG in placenta tissues were analyzed .Results Compared with Group PC, the blood pressure and urine protein of Group HDCP were significantly elevated (P＜0.05).HE staining showed that the decrease in the number of placental vil-li and unclear structure in Group HDCP .The contents of TC, FC, CE, FFA and TG and the expression of FABP4 were al-so significantly elevated in Group HDCP compared with Group PC (P＜0.05).There were significantly positive correla-tions between TC or TG and FABP4 protein level.Conclusion Abnormal lipid metabolism may play an important role in the pathogenesis of HDCP .FABP4 may affect the lipid metabolism , which leads to the occurrence and development of HDCP.
Keywords：hypertension of pregnancySD ratplacenta tissueabnormal lipid metabolismfatty acid binding protein 4
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1441-1444 )