Effect of Astragalus Polysaccharide combined with cisplatin on apoptosis of cervical cancer cells
ZHAI Qiu-li
HU Xiang-dan
XIAO Jing
YU Dong-qing
Abstract：Objective To investigate the mechanism of Astragalus Polysaccharides combined with cisplatin on the apoptosis of cervical cancer cell line siha .Methods Siha cells were divided into four groups : control group, Astragalus Polysaccharide group, cisplatin group and Astragalus Polysaccharide combined with cisplatin group .MTT colorimetric method was used to observe the proliferation of siha cells .The expression of caspase 3, caspase 7, caspase 9 and Bcl-2 in the cells were assessed by Western blot .Results MTT results showed that siha cells were proliferated at 24, 48 and 72 h, and the proliferation of siha cells was significantly inhibited by Astragalus Polysaccharide and cisplatin compared with the control group (P＜0.05, P＜0.01).Compared with Astragalus Polysaccharide and cisplatin alone group , the combination therapy had significant inhibitory effect on siha cells (P＜0.05).The apoptotic rates of Astragalus Polysac-charide group and cisplatin group were significantly higher than that of control group (P＜0.05).The apoptotic rate of Astragalus Polysaccharide combined with cisplatin was significantly increased (P＜0.01) The expression of caspase 3, caspase 7 and caspase 9 protein in Astragalus Polysaccharide group and cisplatin group were significantly higher than those in control group (P＜0.05), and the expression level of Bcl -2 protein was significantly reduced (P＜0.05).The chan-ges of Astragalus Polysaccharide combined with cisplatin were more significant (P＜0.01).Conclusion Astragalus Pol-ysaccharide can induce apoptosis of cervical cancer cell line siha cells in combination with cisplatin .The mechanism of this action may be through the inhibition of Bcl -2 protein expression, thus increasing the caspase9 recruitment, initiation of caspase cascade reaction , further activation of downstream caspase 3, caspase 7 and cell apoptosis .
Keywords：cervical cancersiha cellAstragalus Polysaccharidecisplatinsynergistic effect
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1327-1331 )