MicroRNA-210 inhibits human prostate cancer PC-3 cell apoptosis via silencing of BNIP3

YANG Ning; WU Lei; LIU Jun; WANG Ling

doi:10.3969/j.issn.1001-9448.2018.08.014

Abstract：Objective To observe the expression of microRNA -210 (miR-210) in prostate cancer tissue, and to investigate its effects on the apoptosis of human prostate cancer PC -3 cell and the underlying mechanisms .Methods Quantitative real-time PCR (qRT-PCR) was used to detect miR -210 expression in 30 prostate cancer tissue samples and paired paracancerous samples (n＝20).Moreover, correlations between miR -210 expression and clinical pathologi-cal parameters in prostate cancer patients was analyzed .PC-3 cells were cultured and then transfected with miR -210 mimic/inhibitor or small interfering RNA (siRNA) targeting BNIP3.The expression of miR-210 and BNIP3 mRNA was measured using qRT-PCR.Apoptotic rate and BNIP3 protein expression were assessed by flow cytometry and Western blot, respectively.In addition, bioinformatics and luciferase reporter gene were used to analyze the binding ability between miR-210 and BNIP3.Results The expression levels of miR -210 were significantly increased in prostate cancer tissue compared with paracancerous samples (P＜0.01).Its expression was associated with Gleason score , tumor stages and lymphatic metastasis (P＜0.05), but not with age or smoking history (P＞0.05).BNIP3 was identified as a target gene of miR-210.Transfection with miR-210 significantly inhibited the apoptosis and reduced BNIP 3 mRNA and protein ex-pression in PC-3 cells (P＜0.01).An opposite effect was observed in response to miR -210 inhibitor (P＜0.01).Im-portantly, BNIP3 siRNA pretreatment abrogated the effect of miR -210 inhibitor on PC -3 cell apoptosis (P＜0.01). Conclusion miR-210 is highly expressed in prostate cancer tissues .It can suppress PC -3 cell apoptosis by down -regulating BNIP3 expression.