The effect of ECM-polysaccharide/protein Porous Composites on BMSCs' cartilage differentiationw
LI Shuo-feng
CUI Jing
LIU Guo-liang
HUANG Dong-ming
LI Jing-yi
LIU Bin-yang
ZHAO Xian
CHEN Yi
SUN Hong
Abstract：Objective To construct the extracellular matrix ( ECM)-polysaccharide/protein porous composite scaffold; thus to observe the growth, proliferation and chondrogenic differentiation behavior of bone marrow mesenchymal stem cells (BMSCs) on the scaffold, and the application in engineering cartilage in vitro .Methods Carrageenan, gelati-n and chitosan were used for bionic construction of ECM -polysaccharide/protein porous composite scaffolds .The mor-phology of the scaffolds was observed by SEM .The proliferation of chondrocytes on the scaffolds was detected by MTT .In vitro cartilage tissue formation of BMSCs on scaffolds were shown by type Ⅱcollagen immunohistochemistry staining , using OB as control.Results The scaffolds were porous with three -dimension porous network structure according to SEM ob-servation.The proliferation of chondrocytes on scaffolds was obvious , and there was no significant difference between the experimental group and the control group (P＞0.05).The expression of type Ⅱ collagen was detected in both groups . Conclusion The scaffolds have good biocompatibility and can induce BMSCs differentiate into chondrocytes without OB .
Keywords：cartilage tissue engineeringcomposite scaffold materialcartilage differentiationbone marrow mes-enchymal stem cells
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1137-1141 )