Effects of curcumin on intestinal secretory IgA and oxidative stress in rats with nonalcoholic fatty liver disease
HOU Hong-tao
QIU Yan-mei
FENG Jia
CAI Cong-hui
ZHANG Jian
HU Yi-ting
Abstract：Objective To investigate the effects of curcumin (Cur) on intestinal sIgA and oxidative stress in rats with nonalcoholic fatty liver disease (NAFLD).Methods Thirty male SD rats were randomly divided into normal control group (Group NC), high fat group (Group HF) and Group HF+Cur.High fat diet were given in Group HF group and HF+Cur for 16 weeks.After 8 weeks of high fat feeding in Group HF +Cur, curcumin [200 mg/(kg· d)] were given with lavage for 8 weeks.The pathological changes of intestinal mucosa and liver were observed by optical microscope .The lipopolysaccharide (LPS) of portal vein blood was measured by limulus test .Intestinal sIgA levels were determined by en-zyme linked immunosorbent assay (ELISA).The activity of plasma diamine oxidase (DAO) was determined by spectro-photometric method.The activity of SOD in intestine was measured by hydroxylamine method , and the content of MDA in intestine was measured by colorimetry .The serum levels of alanine aminotransferase ( ALT) and aspartate aminotrans-ferase (AST) were detected by automatic biochemical analyzer .Results Under light microscope , there was no obvious abnormality in small intestinal mucosa and liver in Group NC .In Group HF, intestine mucosa villi were sparse , some of them were broken and defect ; fat droplets infiltration and balloon -like changes in hepatocyte could be seen .In Group HF+Cur, intestine villi were more complete than Group HF , the loss of villi was decreased , and hepatocellular steatosis was significantly reduced.Compared with Group NC, the blood levels of AST , ALT, DAO, and LPS and MDA contents of in-testine in HF group were obviously increased , SOD activity of intestinal and sIgA levels of intestinal were decreased (P＜0.05).Compared with HF group, the blood levels of AST, ALT, DAO and LPS, and MDA content of intestine in Group HF+Cur were significantly lowered; and SOD activity and sIgA level in intestine were significantly elevated (P＜0.05). Conclusion Curcumin can reduce intestinal mucosal barrier injury by increasing sIgA of intestinal mucosa and improving oxidative stress of intestinal mucosa in rats with NAFLD .
Keywords：nonalcoholic fatty livercurcuminintestinal mucosasecretory IgAoxidative stress
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1133-1136 )