The expression of FHL2 in rats with cardiac hypertrophy

FU Zhen-yang; SUN Yi-fan; JIANG Cheng; HUANG Lei; LIN Ji-jin

doi:10.3969/j.issn.1001-9448.2018.08.002

Abstract：Objective To study the expression of FHL2 rats with cardiac hypertrophy .Methods Twenty male Sprague-Dawley (SD) rats were randomly divided into the abdominal aortic constriction group (Group AAC, n＝10) and Sham group (Group Sham, n＝10).The left ventricular hypertrophy model was established by implanting silver U clip on abdominal aorta in Group AAC , the same procedure except for silver U clip implanting was done in Group Sham . Transthoracic echocardiography was performed to detect the myocardial morphology and function 6 weeks after operation . The rats were sacrificed by cervical vertebra dislocation , and the hearts were harvested to measure the weight and analysis the relevant ratio.In addition, the myocardial tissues were detected in morphology with HE staining and Masson staining . The Western blot and real -time quantitative PCR analyze were used to detect the protein expression level and mRNA level of FHL2 in myocardial tissues .Results Compared with the Group Sham , there was a dynamic change of hypertrophy in Group ACC with a significant difference ( P＜0.05).The histological examination also showed significant increasing of cardiac hypertrophy in ACC group (P＜0.05).Both the protein and mRNA levels in Group ACC were significantly higher than those in Group Sham.Conclusion The expression of FHL2 is significantly up-regulated in hypertrophic myocardi-um.FHL2 may play an important role in the development of cardiac hypertrophy .