Effect of liraglutide on the expression of 11beta-hydroxysteroid dehydrogenases Type 1 in the pancreas of insulin resistant rats

LIN Ming-run; LI Wan-gen; WU Miao-jun; XIE Yun-liang

doi:10.3969/j.issn.1001-9448.2018.05.008

Abstract：Objective To observe the effect of liraglutide on the expression of 11beta-hydroxysteroid dehydrogenases Type 1 (11β-HSD1), its cooperative enzyme H6PD and glucocorticoid receptor (GR) in the pancreas of insulin resistant (IR) rats. Methods Forty rats were randomly divided into five groups, normal control group, liraglutide group, food-restricted group, saline group and carbenoxolone group. IR model was established in the latter four groups by being fed with 10％ (w/ v) fructose for 10 weeks. Liraglutide, food restriction, saline and carbenoxolone were given in liraglutide group, food-restricted group, saline group and carbenoxolone group, respectively, for 4 weeks. Body weight and diet were recorded every 3 days. Food-restricted group were fed according to the diet of liraglutide group. At the end of the experiment, weight gain were recorded and triglyceride (TG), fasting insulin (FIns) and fructosamine were assayed. The mRNA and protein levels of pancreatic 11β-HSD1, H6PD and GR were assayed by qPCR and Western blot, respectively. Results Compared with normal control group, serum FIns, TG and weitht gain were significantly increased in the other 4 groups. Compared with saline group, after 4 week-treatment, serum FIns, TG and weight gain were significantly lower in liraglutide group, while the protein levels of pancreatic 11β-HSD1, H6PD and GR were significantly reduced (P ＜ 0. 05). There was no significant difference in 11β-HSD1, H6PD or GR mRNA among the 4 IR groups. Conclusion Liraglutide can improve hyperinsulinemia, hypertriglyceridemia and weight gain in IR rats, and reduce the protein expression of 11β-HSD1, H6PD and GR in the pancreas of IR rats.