Effects of 17-AAG on Migration and Adhesion of Human Choriocarcinoma JAR Cells
CUI Li-jun
LIANG Xiu-jun
XU Qian
LI Yu-hong
XIE Zhao-hui
LIU Lei
HE Ping-ping
XIAO Shuo-yang
Abstract：Objective To investigate the effect of 17-AAG on migration and adhesion of JAR cells. Methods JAR cells were cultured in vitro with 17-AAG at different doses. The inhibitory effects of 17-AAG was measured by MTT assay for calculation of the IC50. Cells migration and adhesion scratches abilities were assessed by cell scratches method and Transwell chamber migration test. The expression of E-cadherin, N-cadherin and β-catenin was assessed by western blotting analysis. Results The results of MTT assay showed that 17-AAG significantly inhibited the proliferation of JAR cells in dose-dependent manner (P ＜ 0. 05). The results of scratches method showed that the coverage rate of the experimental group was significantly lower than that of control group (P ＜ 0. 05). The results of Transwell method showed that the number of cell penetrating cells was decreased in dose-dependent manner compared with control group (P ＜ 0. 05). E-cadherin protein expression was significantly increased in experimental group than control group, while N-cadherin and th theon was significanreduced (P ＜ 0. 05). Conclusion Our results suggest that 17-AAG inhibits JAR cells proliferation. It can inhibit JAR migration and adhesion through mediating EMT signaling pathway by decreasing the expression of N-cadherin and β-catenin protein and increasing the expression of E-cadherin. These results indicate that 17-AAG could act as an molecular target and may serve as a promising therapeutic strategy for JAR.
Keywords：choriocarcinoma17-AAGmigrationadhesion
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 675-679,685 )