Expression of S100A1,lactate dehydrogenase and creatine kinase isoenzyme in the acute myocardial infarction

SHE Ling-zhi; LI Ya-qiong; HE Ling-feng; JING Li; ZHANG Jian-zhong

doi:10.3969/j.issn.1001-9448.2018.04.010

Abstract：Objective To investigate the value of combined detection of S100 calcium binding protein A1( S100A1) , lactate dehydrogenase ( LDH) and creatine kinase isoenzyme ( CK-MB) in the acute myocardial infarction. Methods Thirty autopsy cases were divided into non infarction group, suspicious infarction group and myocardial infarction group. The expression of S100A1, CK-MB and LDH in myocardium was assessed by immunohistochemistry and Western blot. Results In non-infarction group, the coronary artery and myocardial tissue had no obvious lesions. In myocardial infarction group, the coronary artery stenosis was more than 50％ with the histological changes of infarction. In suspicious infarction group, visible coronary artery stenosis more than 50％ but no histological changes of infarction were observed. Immunohistochemical detection showed that the optical density of S100A1, LDH and CK-MB in myocardial infarction group were significantly lower than that of non-infarction group ( P < 0. 05) , In suspicious infarction group, S100A1 and CK-MB optical density values were significantly lower than those in non-infarction group ( P < 0. 05) , while S100A1 and LDH were still higher than those in myocardial infarction group ( P < 0. 05) . In myocardial infarction group, S100A1 protein expression was significantly lower than non-infarction group and suspected infarction group ( P <0. 05) . In myocardial infarction group and suspected infarction group, LDH and CK-MB relative protein contents were significantly lower than those of non-infarction group ( P < 0. 05) . Conclusion The detection of expression of S100A1, LDH and CK-MB in cardiomyocytes is helpful for the diagnosis of acute myocardial infarction.