Establishment of multiple Taqman-MGB probe detection method for drug resistant Mycobacterium tuberculosis
SUN Fei
YI Song-lin
LI Dan
DAI Pei-xi
PENG Fei-wu
TANG Lian-fei
Abstract：Objective To establish a multiple Taqman-MGB probe detection system for drug resistant Mycobacterium tuberculosis. Methods The primers and probes targeting mutation sites of Mycobacterium tuberculosis against tuberculosis first-line drug[rifampicin: RpoB ( 526) /( 531); ethambutol: embB ( 306); isoniazid: KatG ( 315) , inhA(-15); streptomycin: RpsL ( 43)]were designed and synthesized. Wild-type and mutant templates of drug resistant Mycobacterium tuberculosis were constructed and diluted. Drug resistance gene detection reagent of Mycobacterium tuberculosis was prepared, and tested by different templates. 72 tuberculosis samples ( rifampin resistance 20 strains; streptomycin resistance 22 strains; isoniazid resistance 1 strain; multiple drug resistance 17 strains) were analyzed by the reagent. The results were compared with Roche efficacy test. Results The detection system could simultaneously detect 6 tuberculosis mutation sites, and the minimum detection limit was 104 copies /mL. In clinical samples, the total compliance rate was 100％ with good consistency (χ2 =137. 3, P < 0. 001) . Conclusion This study establishes a rapid detection method of drug resistant Mycobacterium tuberculosis, which can be used for health quarantine system and clinical detection.
Keywords：Mycobacterium tuberculosisdrug resistant Mycobacterium tuberculosistaqman-MGBquick detection
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 511-515 )