Effects of cyclical mechanical stretch on the phosphorylation of ezrin in alveolar epithelium
CHEN Wu-tian
DING Ning
Abstract：Objective To investigate the effect of cyclical mechanical stretch on phosphorylaton of ezrin in human alveolar epithelium ( A549) . Methods A549 cells were cultured in type Ⅰ collagen-coated six-well Bioflex elastic silicone membranes and randomly grouped after being identified for follow study. For strain strength experiment ( A549 cells were exposed to cyclic mechanical strain of 5％, 10％ and 20％ for 4 h) and time course experiment ( A549 cells were subjected to cyclic mechanical strain of 20％ for 1 h, 2 h, 4 h, 8 h and 12 h) . Western blot was used to detect the expression of ezrin protein and p-ezrin protein. Results Ezrin and p-ezrin were expressed in A549 cells under normal condition. Compared with control group, cyclic mechanical strain has no significant effects on the expression of ezrin protein. The expression of p-ezrin induced by cyclic mechanical stretch was increased as strain intensified when cells were exposed to 10％ or 20％ strain, but 5％ strain was not enough to induce ezrin phosphorylation. The p-ezrin induced by cyclic mechanical strain was increased in a time-dependant manner. Conclusion cyclical mechanical stretch induces ezrin protein phosphorylation, which may be participated in the alveolus epithelium injury induced by cyclical mechanical stretch.
Keywords：cyclical mechanical stretchalveolar epitheliumezrin proteinphosphorylationlung injury
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 497-500 )