RNA Isolation and identification of serum exosomes from small cell lung cancer patients and the RNA expressional profiles analysis
LIU Li
QIU Fu-man
ZHAO Zhu-xiang
YANG Lei
Abstract：Objective To isolate and identify the exosomes in serum from small cell lung cancer ( SCLC) patients and to reveal the expression profiles of message RNA ( mRNA) and long non-coding RNA ( lncRNA) in them. Methods Exosomes were extracted from serum sample of one case of SCLC patient using the exoRNeasy kit. The morphology and particle size of exosomes were characterized by use of electron microscope and Zetasizer particle size analyzer, respectively. Expression of two landmarks namely CD63 and CD68 was detected by the fluorescently-labeled flow cytometry. Moreover, expression profile was analyzed with the RNA-seq. Five SCLC serum samples were also chosen to validate expressions of four randomly selected lncRNAs. Results Cupping particle could be seen under electron microscopy with uniform particle size ranging from 40 to 130 nm, and the CD63 and CD81 landmarks were observed with positive expression, which were in accordance with the characteristics of exosomes. The transcriptome sequencing detected a total of 1 822 mRNA and 890 lncRNAs. The randomly selected 4 lncRNAs with high expression were further confirmed in another 4 SCLC serum exosomes by qPCR. Conclusion The serum exosomes from SCLC are identified by the morphology, particle size and landmarks. It comprised an abundant of mRNA and lncRNA molecules, which are possibly valuable biomarkers for SCLC prevention and clinical application.
Keywords：small cell lung cancerexosomesRNA-seqlong non-coding RNA
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 359-363 )