Study on the correlation of PAO1 and its mutant strain on the production of antimicrobial peptide LL-37 in macrophages
XIAO Qian
ZHAO Dan
ZHANG Yun-yan
ZENG Jian-ming
LI You-qiang
CHEN Cha
ZHANG Wei-zheng
CAI Ren-xin
Abstract：Objective To study the effects of PAO1 and its mutant strains of Pseudomonas aeruginosa ( PA) on the production of antimicrobial peptide LL-37 in macrophages. Methods Immunomagnetic beads method was applied for isolation of human peripheral blood CD14 + monocytes. Recombinant human granulocyte monocyte set colony stimulating factor was used for the induced-differentiation of macrophages. Different concentrations of PAO1, PA-△lasI, PA-△rhlI and PA-△lasI /rhlI were used to process the macrophages. The enzyme linked immunosorbent assay ( ELISA) method was used for the assessment of antimicrobial peptide LL-37 concentrations produced by PA-induced macrophage. Results Different concentrations of PAO1-, PA-△lasI-, PA-△rhlI-and PA-△lasI /rhlI-induced macrophages began to secrete antimicrobial peptides at 12 h, reached peak at 24 h or 48 h, and gradually declined until disappeared at 72 h, in dose-time-dependent manners. LL-37 was highest in the PA-ΔlasI / rhlI: macrophages =100∶ 1( 1. 631 2 ± 0. 277 0) ng /mL. Conclusion The antimicrobial peptide LL-37 is induced in time-dependent and concentration-dependent manners. Double mutant strain is more conducive for macrophages to produce LL-37, helping PA escape the host immune defense.
Keywords：pseudomonas aeruginosaquorum sensing systemmacrophageantimicrobial peptide LL-37
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 336-339 )