Effect of silencing ADAM17 gene on implanted tumor of MCF-7 breast cancer in nude mice

ZHANG Xiao-bo; PENG Xiao-bing; ZHANG Xue-peng; SUN Ying; JIA Wen-ting

doi:10.3969/j.issn.1001-9448.2018.02.006

Abstract：Objective To investigate the effects of ADAM17 -shRNA on implanted tumor of MCF -7 breast cancer cells in nude mice.Methods MCF-7 breast cancer cells were transfected by lentivirus -ADAM17-shRNA. Thirty nude mice were randomly divided into transfection group(MCF-7-ADAM17-shRNA),vector group(MCF-7-shNC)and control group(MCF-7).The cell suspension were subcutaneously injected in the right side of the footpath of each nude mouse.All nude mice were sacrificed by cervical dislocation on the 26th day after implantation.The morpho-logical structure was observed by HE staining.The expression of ADAM17 and Ki-67 was assayed by IHC,and expres-sion of ADAM17 protein was assessed by Western Blot.Results When nude mice were sacrificed, the tumor sizes in transfection group,control group and vector group were 238.95 ±17.24 mm3,610.48 ±15.96 mm3and 604.50 ±15.14 mm3,respectively.There was significant difference on tumor size among the three groups(F=1 765.86, P=0.00). Compared with the transfection group,there were lager necrosis areas in vector group and control group.The positive ex-pression of ADAM17 was in the cytoplasm,and tumor staining score of ADAM17 in transfection group was 2.45 ±0.44, which was significantly lower than those in vector group(7.09 ±0.18)and control group(7.26 ±0.25)(F=163.13, P=0.00).The positive expression of Ki -67 protein was in the nuclei and the tumor staining score in transfection group was 3.54 ±0.26, which was significantly lower than those in vector group(8.92 ±0.41)and control group(9.28 ± 0.45)(F=481.95,P=0.00).The OD values of ADAM17 protein in control group,vector group and transfection group were 0.632 ±0.037,0.619 ±0.027 and 0.388 ±0.034, respectively.Compared with the vector and control group, the expression of ADAM17 protein was significantly lower in transfection group(F=56.13, P=0.00).Conclusion The growth of human breast cancer MCF -7 cells transfected with ADAM17-shRNA is significantly inhibited in nude mice.