The establishment and biological characteristics of noscapine resistant human colon carcinoma cell line HT-29/Nos,LoVo/Nos and SW480/Nos
HAN Zheng
HUANG Xiao-dong
LIU Meng
ZHU Qing-xi
TAN Jie
Zou Yan-li
HUANG Sha-sha
TIAN Xia
Abstract：Objective To establish the noscapine(Nos)resistanct human colon cancer cell lines and study their biological characteristics.Methods HT-29/Nos, LoVo/Nos and SW480/Nos cell lines were established by stepwise selection in increasing dose of noscapine.IC50of noscapine in HT -29, LoVo, SW480, HT-29/Nos, LoVo/Nos and SW480/Nos cell lines were detected by CCK -8.Cell growth curve was established and the doubling time was accounted. The cell cycle distribution was determined by flow cytometry.The mRNA and protein expression levels of P -gp and MDR-1 in human colon cancer cell and resistant colon cancer cell lines were examined by RT -PCR and Western blot.Re-sults The noscapine resistant human colon cancer cell line HT -29/Nos,LoVo/Nos and SW480/Nos were successfully established,with the resistance indexes of 6.37,7.15 and 6.81,respectively.The doubling times of HT -29/Nos,Lo-Vo/Nos and SW480/Nos were obviously longer than those of HT -29, LoVo and SW480 cell[(51.42 ±2.83)h vs. (35.05 ±3.61)h,P<0.05;(61.93 ±4.22)h vs.(38.38 ±1.88)h,P<0.05; and(48.84 ±1.40)h vs.(31.30 ± 1.75)h,P<0.05)].Flow cytometry demonstrated that the phase G 1was increased and phase S was shortened in drug resistant colon cancer cells.Both mRNA and protein expression levels of P -gp and MDR-1 in HT-29/Nos,LoVo/Nos and SW480/Nos cell lines were higher than those in HT -29,LoVo and SW480.Conclusion We have successfully es-tablished cell line HT -29/Nos,LoVo/Nos and SW480/Nos with Nos resistance,which lays a foundation for the study of resistance mechanism.
Keywords：human colon cancer cellnoscapinedrug resistancebiological characteristics
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 176-180 )