Effect of Ac-SDKP on the expression of CLIC4 in the lungs of rats with silicosis
GUO Di-li
XU Ding-jie
LI Hong-lei
GENG Yu-cong
GAO Xue-min
LI Shi-feng
XU Hong
YANG Fang
WEI Zhong-qiu
Abstract：Objective To investigate the effect of N -acetyl-seryl-aspartyl-lysyl-proline(Ac-SDKP)on the expression of CLIC4 in the process of fibroblast differentiation.Methods Rats were instilled with silica through tra-chea to construct the silicosis model.The expression of α-SMA,collagen type Ⅰand CLIC4 was measured by immuno-histochemistry and Western blot assay.Myofibroblasts induced by TGF -β1 were pre -treated with Ac -SDKP, LY364947,Y-27632,PD98059,SP600125 and SB203580.The expression of α-SMA, collagen type Ⅰ and CLIC4 was assessed by western blot.Results Compared with the corresponding control groups,the expression of α-SMA,col-lagen type Ⅰand CLIC4 was significantly increased in the lung tissues of the silicosis models.Compared with the corre-sponding model groups,after administration of Ac -SDKP,the expression of α-SMA,collagen type I and CLIC4 in the lung tissues was significantly reduced.In addition, Ac-SDKP, LY364947, Y-27632 and PD98059 pretreatment effi-ciently ameliorated the up -regulation of CLIC4, myofibroblast differentiation and collagen deposition in fibroblasts in-duced by TGF-β1.Conclusion Ac-SDKP possibly plays an important role in anti -silicotic fibrosis by inhibiting the CLIC4 signal transduction pathway.
Keywords：N-acetyl-seryl-aspartyl-lysyl-prolinetransforming growth factor -β1CLIC4silicosisfi-brosis
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 171-175 )