The correlation between CaSR and calcium nephrolithiasis formation
ZHU Jia-xing
ZOU Xiao-feng
LIU Quan-liang
LAI Chang-fu
LIU Lin-wei
YANG Zeng-xiang
ZENG Qing-ming
ZENG Xiao-peng
QIU Kai-yang
WANG Liang
NIE Xiao-ming
XIE Tian-peng
ZHANG Guo-xi
Abstract：Objective To investigate the expression of calcium sensing receptor(CaSR),receptor activity modif-ying protein(RAMP),caveolin and filamin A(FLNA)in kidney tissue of patients with calcium nephrolithiasis(CN), and to explore the correlation between CaSR and CN.Methods From March 2016 to May 2017,30 cases of no-func-tion kidneys were collected after the nephrectomy as the case group,and 30 cases of normal renal tissues far away from the tumor were collected as the control group after the radical nephrectomy.CaSR, caveolin, RAMP and FLNA mRNA ex-pression was assessed by Real -time PCR.Expression of CaSR, caveolin, RAMP and FLNA protein was assessed by Western blot and immunohistochemistry(SABC).Results In case group,the mRNA expression and the protein expres-sion of caveolin, RAMP and FLNA were significantly higher(P <0.05), while CaSR was significantly lower(P <0.05),compared with control group.Spearman correlation analysis showed the expression of CaSR protein was significant -ly negatively correlated with caveolin,RAMP and FLNA(P<0.05).Conclusion The down-regulation of CaSR ex-pression may promote the formation of CN;and caveolin, RAMP and FLNA may be involved in the formation of CN via regulating the expression of CaSR.
Keywords：CaSRnephrolithiasisstone formation
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 92-96 )