The expression and clinical significance of senescence markers in colorectal adenoma and colorectal cancer tis-sues

XIE Dan-yang; WU Bin-wen; LI Dong-feng

doi:10.3969/j.issn.1001-9448.2017.24.026

Abstract：Objective To investigate the expression of the senescence markers , including the senescence -asso-ciated βgalactosidase (SA-β-gal) and P16INK4A, in colorectal adenoma and colorectal cancer tissues , as well as their clinical significance .Methods SA-β-gal staining and immunohistochemical staining was used to evaluate the expres -sion of SA-β-gal and P16INK4A in 30 cases of paired colorectal adenoma and normal tissues , 48 cases of paired colorectal cancer and normal tissues .The expression of SA -β-gal and P16INK4A in different pathological characteristics of colorectal adenomas and colorectal cancer were analyzed .Results The expression of SA-β-gal and P16INK4a in colorectal adeno-ma tissues was significantly higher than the colorectal cancer tissues and normal tissues adjacent to adenoma , and the ex-pression of SA-β-gal and P16INK4a in the colorectal cancer tissues was significantly lower than normal tissues adjacent to cancer.The expression of SA -β-gal was low in the villioustublar adenoma , the colorectal adenoma with <1cm size, and advance adenoma .The expression of SA-β-gal was also low in low differentiated adenocarcinoma , the colorectal with distant metastasis, and the Stage IV colorectal cancer .The expression of P16INK4a was high in the colorectal cancer without lymph node metastasis, and Stage ⅠandⅡcolorectal cancer.Conclusion The expression of SA-β-gal and P16INK4a show that senescence may be as inhibition pathway involved in the process of the colorectal adenoma turning into cancer . The detection of SA-β-gal is supposed to be a marker to find high -risk precancerous lesions, and P16INK4a is supposed to be as application indicating good prognosis .