Oxidative stress induces renal tubular epithelial cells necrosis in a cyclophilin D-Dependent Manner
YANG Huan
LI Rui-zhao
DONG Wei
ZHANG Shu
LIU Shuang-xin
LIANG Xin-ling
Abstract：Objective To investigate the role of cyclophilin D ( Cyp D ) on oxidative stress -induced necrosis in renal tubular epithelial cells .Methods Eighteen male Wistar rats were randomly divided into Control group , Sham con-trol group and Ischemia reperfusion injury group .HE staining was carried out and serum creatinine was measured .H2 O2 was used to induce oxidative stress by different times or concentrations .Cyp D-siRNA or CsA were applied as pretreat-ment .The manner of cell death was identified by flow cytometry .Results Serum creatinine was significantly increased in Ischemia reperfusion injury group than those in Control group and Sham control group (P=0.017, P=0.018).Proximal renal tubulars expansion , loss of brush border , and a large number of tube type and cell vacuoles degeneration were ob-served in Ischemia reperfusion injury group by HE staining .H2 O2 induced HK-2 cells necrosis in dose -and time-de-pendent manners, which was inhibited by Cyp D -siRNA and CsA (P=0.026, P=0.008).Conclusion Cyp D plays an important role in oxidative stress -induced necrosis , which could be ameliorated by inhibiting Cyp D .
Keywords：cyclophilin Drenal tubular epithelial cellnecrosisacute kidney injuryoxidative stress
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 3784-3787,3791 )